New DNA based assay rapidly identifies E. coli
My first paper just got published in this month’s issue of the Journal of Clinical Microbiology. We, primarily researchers at Children’s Hospital of Pittsburgh, developed an assay using a technique called Loop Mediated Isothermal Amplification (LAMP) to identify E. coli in a sample, ranging from blood to urine, without the need to first extract the DNA or to use a PCR machine. Rather, a sample can be added directly to a reaction mixture and heated on a heating block for 30-60 minutes. The sample will change color and fluoresce under UV light, allowing a positive result to be quickly and easily identified. We hope that this technique can be used in out patient clinics and clinics in developing countries to identify E. coli infections such as a UTI (urinary tract infection).
Further research is currently being conducted to develop these rapid tests for other infectious agents and bring them into clinical use.
Think Gene at Technorati
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